Intestinal epithelial cells play an important role in the mucosal immune and inflammatory reactions via the expression and secretion of proinflammatory cytokines such as interleukin-6 (IL-6) and interleukin-8 (IL-8). The expression of both interleukins is regulated by nuclear factor kB (NFkB). Phytic acid (IP6) is an essential component of high fiber diet. It is physiologically present in the human large gut at concentrations reaching 4 mM. It exhibits pleiotropic health beneficial effects including anti-oxidant and anti-tumor activities. Recent studies showed that IP6 can modulate immune functions of intestinal epithelium through regulation of proinflammatory cytokines secretion.

The aim of this study was to examine the role of IP6 in immunological function of intestinal epithelial cells by analyzing its effect on the expression of IL-6 and IL-8 genes by IL-1β-stimulated colonocytes Caco-2. It was also evaluate the influence of IP6 on the expression of genes encoding p50 and p65 subunits of NFkB and that of its inhibitor IkBα in by cells stimulated with IL-1β. A kinetic study of mRNAs expression in cells was performed after their treatment with 1 and 2,5 mM IP6 for 3, 6 and 12h. Quantification of the genes expression was carried out using real time QRT-PCR technique.

Treatment of cells with IP6 resulted in a strong decrease in both IL-6 expression (at 3h and 6 h) and IL-8 expression (3h). IP6 at all used concentrations had no influence on transcription of p65 and p50 genes in Caco-2 cells stimulated with IL-1β, however, they expressed higher level of the inhibitor IkBα in response to 2,5 mM IP6 treatment .

The results of these studies suggest that IP6 may exert immunoregulatory effects on intestinal epithelium by influencing transcriptional activity of genes encoding inhibitor IkBα and proinflammatory cytokines IL-6 and IL-8.

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