Protective ointments containing chelating agents are often used by sensitized subjects to prevent the contact allergy dermatitis by chemical binding of allergenic metal ions. The aim of the study was to evaluate the influence of the aminoacid (Gly, Asp, His) ingredient of the protective ointments with Na₂H₂EDTA based on the two formulas (eucerin or hascobase) on the chelation ability of Ni²⁺ and Co²⁺ ions. The in vitro test with the diffusion chamber consisting of donor and receptor compartment separated by artificial membrane of chemically modified cellulose was applied. The volume of receptor compartment was filled with the tested ointment and the surface of the membrane was washed with the solution containing Co²⁺ or Ni²⁺ dissolved in water. The concentration of Ni²⁺ and Co²⁺  in the solution was determined by atomic absorption spectrophotometry (AAS vario) after every hour of the  extraction. Statistical significance of the observed differences in chelation ability of the analyzed protective ointments was confirmed by analysis of variance. The experiments were run for 5 h. The study showed that the protective ointment containing 10% Na₂H₂EDTA and histidine had the most effective Ni²⁺ and Co²⁺ chelation properties. A histidine of 10% concentration in the ointment was optimal for metal ions binding. Pharmaceutical formula (eucerin or hascobase) of the ointment had no influence on the chelation ability of chosen metal ions.

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