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Electrodeposition method for preparing SERS- and LSPR-active platforms
|Marta Siek 1, Agnieszka Kamińska 1, Marcin Łoś 2, Joanna Niedziolka-Jonsson 1|
1. Institute of Physical Chemistry Polish Academy of Sciences (IPCPAS), Kasprzaka 44/52, Warsaw 01-224, Poland
| Novel biosensing methods, including Surface Enhanced Raman Spectroscopy (SERS) and Localized Surface Plasmon Resonance (LSPR), involves using of noble metal nanoparticles. The signal detected depends on nanoparticles size and shape, interparticle distance and refractive index of surrounding media. Many attempts have been made to produce cheap, efficient and reproducible substrates.[2-6]|
We present a universal biosensing platform for virus or antibody label-free recognition. It was produced using electrodeposition of silver nanoparticles onto tin-doped indium oxide (ITO) by cyclic voltammetry. Our method is consistent with the principles of green chemistry. Morphology of substrates was changed with tuning the scan rate of the applied potential and the number of scans. The platform is suitable both for surface enhanced Raman spectroscopy (SERS) and localized surface plasmon resonance (LSPR). The obtained substrates are reproducible and stable. Efficacy of our AgNPs substrate for biosensing was demonstrated for the detection of neurotransmitters such as choline at low concentration and with short detection time at SERS and for detection of specific binding between avidine and biotinylated bacteriophages at both SERS and LSPR.
 B. Sepúlveda, P.C. Angelomé, L.M. Lechuga, L.M. Liz-Marzán, LSPR-based nanobiosensors, Nano Today, 4 (2009) 244-251.
Presentation: Short communication at SMCBS'2011 International Workshop, by Marta Siek
See On-line Journal of SMCBS'2011 International Workshop
Submitted: 2011-09-05 11:51 Revised: 2011-10-21 14:58