The development of new and stable gene carrier is of great importance to improve the tranfection efficiency and achieve to certain fundamental needs in gene therapy such as therapeutic protein formation, immunogenicity enhancement and apoptosis induction. The stability and applicability of the system which contains single-walled carbon nanotube (SWNCT) to combine with certain sequence of plasmid DNA(EGFp-C1) were examine by computer simulation. Raman spectroscopy was used as supporting information to verify the process and phenomenon of complex formation. Two diamine molecules, 1,4-diaminobutane and polyoxyethylene bis-amine, were applied in order to load positive charges onto the surface of SWCNT under physiology condition and also enhance the binding ability of CNT to EGFp-C1. The formation of peptide bond was examined with FTIR to confirm the success crossliking. The amount of surface functional groups was checked out with TGA, which found that they are at an extremely low concentration but show great influence on the physical property. Biocompatibility was evaluated with LDH, MTT and total DNA. The results show a significant increase in cell viability and number for SWCNT-1,4-diaminobuatne complex. IL-6 release was measure to justify the inflammatory inducing and give the hint of translocation mechanism. The binding efficiency of functionalized SWCNT to EGFp-C1 was analyzed through the binding strength under electrophoresis.In-vitro transfection was also done to justify the transfection efficiency but showing no significant increase compare with other non-viral vectors which may due to the binding force of such complex.

Keywords: gene carrier, single-walled carbon nanotube, 1,4-diaminobutane, polyoxyethylene bis -amine, biocompatibility

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