Preliminary studies on application of library of artificial receptors for differentiation of metabolites in urine of healthy and cancer mice

Małgorzata E. Walczak 1Justyna Frączyk 1Zbigniew J. Kamiński 1Joanna Wietrzyk 2Beata Filip-Psurska 3

1. Technical University of Łódź, Institute of Organic Chemistry (PŁ), Żeromskiego 116, Łódź 90-924, Poland
2. Polish Academy of Sciences, Institute of Immunology and Experimental Therapy (IITD), Rudolfa Weigla 12, Wrocław 53-114, Poland
3. IITD PAN, Wrocław, Weigla 12, Wrocław 53-114, Poland

Abstract

Metabolites are small molecules necessary for the maintenance, growth and normal function of a cell. The term metabolome refers to the complete set of metabolites in an organism. Metabolomics and metabonomics cover all techniques to identify and quantify all metabolites in a biological system, as well as the monitoring of changes in the metabolome of a biofluid, cell culture or tissue [1]. Metabolomics carries a huge potential for early diagnosis, therapy monitoring and for understanding the pathogenesis of many diseases [2]. Despite of the extensive progress in this field, there is still a great need for development of new non-invasive diagnostic methods. The microarrays have emerged as one of the most prominent and revolutionary technologies currently available for multiplexed detection. Peptide microarrays, usually synthesized by SPOT technology, are gaining prominence as a vital tool for high-throughput screening. This techniques are most commonly applied in epitope-mapping, substrate profiling and probing peptide-ligand interactions [3].

In our studies, we tried to apply the new type of microarrays formed by self-organization of N-lipided peptides immobilized on the cellulose for systematic searching the differences in the composition of the body fluids of healthy and malignant tumor bearing mice. The arrays of N-lipidated peptides are prone to formation of monolayer of highly organized structures able to molecular recognition. These structures very efficiently recognize the shape, size, polarity of ligands [4]. As the recognition of ligands depends on the structure of immobilized peptides fit is assumed that appropriately designed peptidic fragments allow selective binding of cancer markers from body fluids. In this preliminary studies urine was used and the different profile of binding was observed for healthy and malignant tumor bearing mice.

Acknowledgement:

This work was supported by Ministry of Science and Education Poland N N 405 669540.

 

References:

[1] Barderas M.G.; Laborde C.M.; Posada M.; de la Cuesta F.; Zubiri I.; Vivanco F.; Alvarez-Llamas G.; J. Biomed. Biotech., 2011,doi:10.1155/2011/790132.

[2] Gowda G.A.; Zhang S.; Gu H.; Asiago V.; Shanaiah N.; Raftery D.; Expert Rev Mol Diagn., 2008, 8, 617-633.

[3] Reineke U.; Volkmer-Engert R.; Schneider-Mergener J.; Curr. Opin. Biotechnol., 2001, 12, 59–64

[4] Frączyk, J.; Malawska, B.; Kamiński, Z.J., J. Comb. Chem., 2009, 11, 446-451.

Frączyk, J.; Kolesińska, B.; Czarnecka, A.; Malawska, B.; Więckowska, A.; Bajda M.; Kamiński, Z.J., QSAR & Comb. Sci. 2009, 11; 728-736.

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Presentation: Poster at IX Multidyscyplinarna Konferencja Nauki o Leku, by Małgorzata E. Walczak
See On-line Journal of IX Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2014-03-15 12:21
Revised:   2014-05-02 19:28
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