THE CYTOTOXIC GLYCOSIDES OF INDOLO[2,3-B]QUINOLINE DERIVATIVES. IN VITRO AND IN VIVO STUDIES

Aleksandra Nowicka 1Małgorzata Piskozub 1Joanna Wietrzyk Katarzyna Badowska-Rosłonek 2Łukasz S. Kaczmarek 2Wanda Peczyńska-Czoch 3

1. Polish Academy of Sciences, Institute of Immunology and Experimental Therapy (IITD), Rudolfa Weigla 12, Wrocław 53-114, Poland
2. Pharmaceutical Research Institute (IF), Rydygiera 8, Warszawa 01-793, Poland
3. Wrocław University of Technology, Department of Chemistry, Wybrzeże Wyspiańskiego 27, Wrocław 50-370, Poland

Abstract

The cytotoxic activity in vitro of glycosides of indolo[2,3-b]guinoline having daunosamine or acosamine moiety was evaluated. In our research we used human colon cancer (LoVo), uterine sarcoma (MES-SA), promyelocytic leukemia (HL-60), lung cancer (A-549) and melanoma (Hs294T) cell lines. We found that all the indoloquinolines studied were cytotoxic against the cells of all cancer lines used. They showed the highest cytotoxic activity against human leukemia cell line (HL-60) and the lowest against the human melanoma cell line (Hs294T) as well as against the human colon cell line (LoVo) (Table1.)

tabelka1.GIF

wzory.GIF

To define antitumor activity the compound IBR-5 in vivo we applied mouse leukemia cell line (P-388). The results of our research show toxicity of studied compound in higher range of doses (30, 50 and 100 mg/kg). The average survival time (AST) of mice, which was administered with the compound IBR-5 in higher doses was 7 days and for control mice 12 days. Additionally in lower range of doses (10, 5, 1 mg/kg) the compound did not show antitumor effect. The average survival time of mice which were administered with the compound in lower doses was comparable to AST of control mice.

 

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Presentation: Poster at V Multidyscyplinarna Konferencja Nauki o Leku, by Aleksandra Nowicka
See On-line Journal of V Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2006-02-01 07:30
Revised:   2009-06-07 00:44