The cytotoxic activity of glycosides of indolo[2,3-b]quinoline derivatives.

Katarzyna Badowska-Rosłonek 1Agnieszka Ciesielska 1Marta Świtalska 2Joanna Wietrzyk 2Łukasz S. Kaczmarek 1Wanda Peczyńska-Czoch 3

1. Pharmaceutical Research Institute (IF), Rydygiera 8, Warszawa 01-793, Poland
2. Polish Academy of Sciences, Institute of Immunology and Experimental Therapy (IITD), Rudolfa Weigla 12, Wrocław 53-114, Poland
3. Wrocław University of Technology, Department of Organic Chemistry, Wybrzeże Wyspiańskiego 27, Wrocław 50-370, Poland

Abstract

The cytotoxic activity in vitro of glycosides of  indolo[2,3-b]guinoline ( 5H and 6H series) were tested against cervix carcinoma cell line (KB).

The cells were placed in 96-well flat-bottom plates at a density of 1x104 cells per well in 100 ml of cultured medium 24 hours before addition of the tested compounds. The cells were exposed to the test compounds at concentrations 0.1, 1, 10, and 100 mg/ml for 72 h. The in vitro cytotoxic effect of all agents was examined using the SRB assay.

We found that all tested indoloquinolines showed cytotoxic activity against KB cells and the most potent were two of them: IBR-15 (ID50: 0,29±0,12 mg/ml) and IBR-17 (ID50: 0,33±0,08 mg/ml). The  5H serie was more active against cervix carcinoma cell line than  6H one.

 

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Presentation: Poster at VI Multidyscyplinarna Konferencja Nauki o Leku, by Marta Świtalska
See On-line Journal of VI Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2008-04-07 10:58
Revised:   2009-06-07 00:48