Rate of Binding of Host Molecules to Artificial Receptors Formed by Self-Organisation of Lipidated Oligopeptides

Justyna Majchrzak ,  Justyna Frączyk ,  Zbigniew J. Kamiński 

Technical University of Łódź, Institute of Organic Chemistry (PŁ), Żeromskiego 116, Łódź 90-924, Poland

Abstract

The arrays of artificial receptors formed by N-lipidated oligopeptides immobilized, in the highly ordered fashion, on the solid support were synthesized and used in the studies on molecular recognition. Thus, even in the case, when the single receptor in a differential array does not necessarily have selectivity for a particular analyte [1], the combined fingerprint response can be extracted as a diagnostic pattern visually, or using any chemometric tools.

The previous studies confirmed that alternation of the binding pattern could detect even tiny structural changes of guest molecules and therefore offer a new tool for SAR studies. An assay of physiological fluids and tissue homogenates has been found useful for diagnosis of thyroid tumors [2].

Further successful application of arrays of artificial receptors for profiling of metabolome depends on efficiency of competitive binding of guest molecules and colored indicator dye. Therefore we attempted to measure the rate of binding of coloured ligands in the search for the most efficient reported dye.

The rate measurements were performed by spectrophotometric method using 96 wells ELISA plate reader.

Acknowledgements: The study was supported by the Polish State Committee for Scientific Research under the Project 2 P05F 03130.

[1] Wright A.T.; Anslyn, E.V. Chem. Soc. Rev., 2006, 35(1), 14-28.
[2] Balcerzak, W.; Bednarz, W.; Domoslawski, P.; Olewinski, R.; Kolesinska, J.; Kaminski, Z.J.; Dziarkowska, K.; Wieczorek, P. Polish J. Endocrin. 2006, 57(4), 308-313.

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Presentation: Poster at VI Multidyscyplinarna Konferencja Nauki o Leku, by Justyna Frączyk
See On-line Journal of VI Multidyscyplinarna Konferencja Nauki o Leku

Submitted: 2008-03-15 09:14
Revised:   2009-06-07 00:48
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