Macrophages, being the main source of the reactive oxygen (ROS) species in human, are of the prime importance for inflammatory response. Catalase is an antioxidant enzyme protecting living organisms against negative action of ROS. Recently a number of reports have been focused on the fluoride involvement in a respiratory processes and subsequent ROS synthesis. Fluoride is proved to both activate and inhibit activity of various enzymes.

The aim of this study was to examine the inhibitory effect of sodium fluoride (NaF) on the macrophage catalase (Cat) activity over a range of NaF concentration and time.

Material and methods: The experiment was conducted on the macrophages obtained from the THP-1 monocytic cell line. Macrophages were incubated with NaF used at concentration of 0,5 mM, 1 mM, 5 mM. The inhibitory effect of each NaF concentration was examined over a range of incubation time (10, 30 or 60 min). Catalase activity was estimated by spectrophotometrically. The protein concentration was measured by Bradford method. For related samples significance was first checked with ANOVA-Friedman and Wilcoxon matched-pair tests. p<0,05 was considered significant.

Results: NaF used in the experiment exerted statistically significant inhibitory effect on the catalase activity over a range of concentration and time (p<0,05). Inhibition of catalase activity by NaF used at the concentration of 0,5 mM was significant after 30 (p<0,05; n=6) and 60 minutes (p<0,05; n=6) if compared to control. Addition of 5 mM NaF significantly reduced enzyme activity after 10 (p<0,05; n=6), 30 (p<0,05; n=6) and 60 minutes (p<0,05; n=6) of incubation if compared to control. Incubation with 1mM NaF resulted in significant inhibition of catalase activity after 10 (p<0,05; n=6) and 60 minutes (p<0,05; n=6).

Conclusion: Fluoride inhibited catalase activity in macrophages. Inhibitory effect depended on both NaF concentration and on the time of macrophages incubation with NaF. These results suggest NaF involvement in impairment of antioxidant systems actions and reduced cell protection against reactive oxygen species action.   

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