The development of resistance to multiple drugs used on cancer chemotherapy is a serious limitation of this form of treatment. In resistant cells there is correlation between decrease drug accumulation and expression of a 170kD cell surface glycoprotein which is designate P-glycoprotein, encoded by the MDR1 gene and 190KD glycoprotein - multidrug resistance protein - MRP1.

There is evidence that the MRP1 gene encodes a human GS-X pump. MRP1 contains multiple transmembrane domains plus two intracellulary localized ATP - binding cassettes, suggesting that it functions as an efflux pump for drug elimination, including doxorubicin.

Cancer cell resistance to antitumor agents has often been found associated with increases in intracellular glutathione (GSH) level.

In the present study we investigated the effect of doxorubicin on GSH level in human melanoma cells, sensitive and resistant. The GSH content was measured by BIOXYTECH^® GSH/GSSG - 412^TM Kit.

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