INTRODUCTION

There are many reports on antioxidative properties of flavonoids presented in Radix Scutellariae baicalensis Georgi. The most active are baicaline and baicaleine. There are also some investigation on antioxidative properties of Antoxid (AX), the water-alcoholic extract obtained from Radix Scutellaria baicalensis in crystalline form, standardized on baicaline. There are no reports on Antoxid utility in oxidative stress caused by chemicals e.g. environmental aromatic hydrocarbons.

The aim of study was the investigation of AX influence on lipid peroxidation stimulated by the chemicals: t-butyl peroxide or xylene. The results were compared with vitamin C activity.

EXPERIMENTAL

The study was performed on in vitro model, human placental mitochondria. The mitochondria were isolated by Radi method from mature placenta obtained after physiological delivery from Medical University Clinic. The proteins in mitochondria were measured by Lowry method. The AXwas dissolved in mitochondrial buffer (TRIS-HCl - pH-7,4) and used in following concentrations: 1,5; 3,0; 6,0; 12,0 and 30 µg/ml. The lipid peroxidation was evaluated by malondialdehyde level measured spectrophotometrically with thiobarbituric acid method (TBARS).

RESULTS AND DISCUSSION

At first the antioxidative properties of AX were examined at mitochondria stimulated with 1% t-BOOH. It was observed that AX inhibits lipid peroxidation in three from five concentration - in dose 6,0; 12,0 and 30 µg/ml but not in dose 1,5 and 3,0 µg/ml. It means that AX in doses higher than 6 µg/ml is able to reduce such free radical process as lipid peroxidation leading to MDA level decrease. The decrease of MDA level was dose dependent (p<0,001).

The aim of study was to examine the utility of AX in oxidative stress stimulated by environmental chemicals like aromatic hydrocarbons, for example xylene. The next experiment was performed in order to explain whether simultaneous exposition to xylene and AX doesn't give harmful interaction in free radicals process. The results show that simultaneous mitochondria treatment with xylene in conc. 17,64 µg/ml and AX in conc. 6,0 or 12,0 µg/ml leads to statistically significant (p<0,001) decrease in MDA level in comparison to the control without AX. The obtained results points at high effectiveness of AX in reduction of lipid peroxidation stimulated by aromatic hydrocarbon-xylene.

It was also interesting to explain whether the effectiveness of AX depends on time of toxic action. It means whether AX is more useful as preventing agent (giving before the exposition to hydrocarbon) or repairing agent (giving after the exposition to hydrocarbon). In order to examine the repairing effect, AX in conc. 6,0 or 12,0 µg/ml was added to mitochondria incubated with xylene in dose 17,64 µg/ml 30 min. after the exposition. The results show that only higher dose (12,0 µg/ml) was able to reduce MDA level significantly (p< 0,001) (ryc. 3). Different results were obtained when AX was given 30 min before exposition to xylene. Preincubation of mitochondria with AX in conc. 6,0 or 12,0 µg/ml successfully prevents MDA level increase.

CONCLUSIONS

1. Antoxid in conc. >6 µg/ml significantly inhibited lipid peroxidation caused by t-BOOH or xylene.

2. The effectiveness of Antoxid depends on time of exposition.

3. Antoxid given before or simultaneously with xylene is more active than given after xylene.

4. Antoxid has preventing and repairing activity, but acts stronger as preventive agent.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/6546 must be provided.

1. Role of estradiol in chromium-induced oxidative stress
2. New form of coenzyme Q10 (PureSorb-Q^TM40) inhibits free radicals processes caused by cisplatin.
3. Baicalin inhibits free radicals processes caused by chromium and cisplatin.
4. The role of estradiol in reducing the ethanol toxicity
5. THE RESEARCH ON BIOLOGICAL ACTIVITY OF ANTOXID. THE INFLUENCE ON HYDROXYL RADICAL GENERATION.
6. THE RESEARCH ON BIOLOGICAL ACTIVITY OF ANTOXID. THE SYNERGISTIC EFFECT OF VITAMIN C AND ANTOXID ON FRAP DEPENDS ON THE RATIO OF COMPONENTS.
7. THE RESEARCH ON BIOLOGICAL ACTIVITY OF ANTOXID. PART I. THE INFLUENCE ON FERRIC REDUCING ANTIOXIDANT POWER (FRAP)