INTRODUCTION

Our previous investigation showed the stronger effect of Antoxid than vitamin C on ferric reducing plasma ability (FRAP). Antoxid is the water-alcoholic extract obtained from Radix Scutellariae baicalensis Georgi according to special procedure. The final step in preparation was the crystallization. Antoxid is the main component of BAICADENT gel used in stomatology. It looks that the most important components which determine the FRAP activity of Antoxid are flavonoids baicaleina and baicaline.

The aim of presented study was to examine the joint effect of Antoxid with vitamin C. It is interesting to know whether the common use of those two agents gives harmful interaction or brings some advantages.

The material was blood plasma from patients of Surgery Dept. in Medical University Clinic. The blood was taken on anticoagulant. The patients didn't receive any drugs. FRAP was evaluated by the measurements of Fe⁺²/TPTZ-complex by colorimetric method with spectrophotometer. The three combinations of vit. C (C) and Antoxid (A) mixture were used for examination: M1 (5 µg/ml A + 5 µg/ml C); M2 (15 µg/ml A + 5 µg/ml C); M3 (5µg/ml A + 15 µg/ml C).

The results were evaluated with t-Student test.

Every mixture (M1, M2 and M3) statistically significantly increase the value of FRAP (p=0.02 - 0.002). It looks that the mixture of Antoxid and vitamin C in various concentrations posses antioxidative properties. There is no doubt that the most advantageous is the combination of low doses of agents in stechiometric ratio (1:1). The Antoxid in dose 5 µg/ml didn't show the influence on FRAP. The influence of 5 µg/ml vitamin C on FRAP was also very weak. The mixture of Antoxid in concentration 5 µg/ml with 5 µg/ml vitamin C showed strong synergistic effect on FRAP increasing its value significantly (p=0.00002). There are some observation that the presence of flavonoids and vitamin C in diet is very useful specially in the prophylaxis of cardiac diseases.

CONCLUSIONS

1. The mixture of Antoxid and vitamin C shoved antioxidative properties measured by increase of human plasma FRAP value in various concentrations.

2. The synergistic action of components was observed for stechiometric ratio of Antxid with vitamin C.

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