INTRODUCTION

Antoxid is a new herbal free radical scavenger obtained from Radix Scutellariae baicalensis Georgi. The water-alkoholic extract of Radix obtained according to described procedure is very rich in flavonoids with baicaline as a main one (72% approximately). Antoxid is the main component of BAICADENT gel used in stomatology. There are several parameters reflecting the antioxidative properties of agents in blood. One of them is FRAP (Ferric Reducing Ability of Plasma). However the extract from Scutellariae baicalensis, standarised on baicaline, was a subject of various investigations, particularly evaluating the antioxidative properties, there were no reports on its influence on FRAP.

The aim of study was to determine the influence of Antoxid on ferric reducing ability of plasma (FRAP) and the comparison of the results with vitamin C influence on FRAP.

EXPERIMENTAL

The material was blood plasma from patients of Surgery Dept. in Medical University Clinic. The blood was taken on anticoagulant. The patients did not receive any drugs. FRAP was evaluated by the measurements of Fe⁺²/TPTZ-complex by colorimetric method with spectrophotometer. The Antoxid was dissolved in methanol/water and used in concentration as follow: 5,0; 10,0; 20,0; 30,0 and 50,0 µg/ml.The results were evaluated with t-Student test.

RESULTS AND DISCUSSION

The comparison of Antoxid ferric reducing capacity with plasma ferric reducing capacity showed in general lower activity of Antoxid than plasma. However the date of FRAP for plasma and Antoxid in concentration 20-50 µg/ml were close to each other. The Antoxid in lower concentration (5-10 µg/ml) had much lower ferric reducing ability (FRAP) than plasma.

The examination of Antoxid influence on plasma ferric reducing capacity showed strong antioxidative ability of extract. Antoxid in concentration 10-50 µg/ml statistically significant (p=0.000016) increased FRAP. The strongest effect was obtained with 30 µg/ml concentration of Antoxid.

The antioxidative properties of various flavonoids are well known. They are good chelators of metals ions, particularly iron or cuprum metals, Fenton reaction catalyzer. The antioxidative properties of baicaline are connected with xanthine oxidase inhibition.

Flavonoids influence also free radical generation by chelating transition metal ions, which catalyze the reaction. It is reported that high flavonoid intake shows significant effect on the liver, but not on the brain. It is probably because the liver is the main metabolic organ of flavonoids. Many flavonoids have hepato-protective effect when the liver is under pathological condition.

Recently Firuzi examined in vitro the influence of various flavonoids on FRAP, using the artificial model of blood (TPTZ/FeCl₃). The most active was baicaleine. It looks that it plays more important role in FRAP influence than flavonoid baicaline. So it seems that the Antoxid's ferric reducing ability is determined mainly by baicaleine with the participation of baicaline.

CONCLUSIONS

1. Antoxid in conc.> 5 μg/ml increases ferric reducing ability of human plasma.

2. Antoxid in conc. 30 μg/ml is more active than vitamin C in influence on FRAP.

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