Bacteriophages are viruses that infect bacterial cells. They are widely spread in nature occupying different environmental niches. Phage lysate, which is received by multiplication in Gram-negative bacteria include bacteria  cell ingredients such as lipopolysaccharides (endotoxins). Lipopolysaccharides are released from  bacteria during cell lyses or multiplication. Released endotoxins induce a strong response of immune system influencing on activation of monocytes and macrophages, they also play a central role in the pathogenesis of septic shock. Biological preparations used in therapy should be endotoxin-free. The threshold level of endotoxin for  intravenous application is 5 endotoxin units (EU) per kg body weight and hour (European Pharmacopoeia 1997) and for breath ≈ 200 EU m^-3 [1].
The present study proposes  a new method for endotoxin removal from bacterial lysates. The heterophase extraction technique to higher alcohols such as 1-octanole and 1-buthanole went after addition Mg²⁺ ions to bacteriophage lysate. Escherichia coli B were cultured in medium based on casein acid hydrolysate. The preparation of bacteriophage lysate was done in fermentor New Brunswick BIOFLO 415. Phage lysate after multiplication  had   33 600 EU/ml and at  the end of the purification process  1 EU/ml. The yield of endotoxin removal process was over 99% and the phage lytic activity loss was limited to one range. These method is scalable to technological use. These work was supported by NCBiR 13-0089-06.

[1] Hasday, , J., Dubin, W., Fitzgerald, T., Bascom, R.. Cigarettes are a rich source of bacterial endotoxin. CHEST 109 (1996) 63S-64S.

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