The objective of the paper was: modification of amoxicillin extraction method in serum of pigs and poultry, identification and comparison of amoxicillin pharmacokinetic parameters in pigs and poultry that will facilitate better use of antibiotic with variation in dosage.

The study was carried out on 9 piglets of Polish breed “Polska Biała Zwisłoucha” and on 35 chickens of “Hubart Evolution” breed. The piglets were given drug tested in one dose per os in doses 28 mg/kg body weight as calculated for amoxicillin.  The drug was dissolved in water. Blood was sampled from the external jugular vein during after 1, 2, 4, 6, 8, 10 hours of drug administration. The birds were given the drug orally in equal dose of 20 mg/kg of body weight as calculated for the active substance. Blood samples were sampled in the course of exsanguinations when theanimals were pharmacologically deprived of consciousness, after 0,5,  1, 2, 3, 4, 6, 8 hours from the administration of the preparation. The assays performed in the blood serum of the piglets with the HPLC technique proved that minimal concentrations of amoxicillin were present even after 10 hours from the last dose of the drug. The curve depicting the course of amoxicillin concentrations in the drug tested preparation, had an ascending and descending course with the highest level after 2 hours from the veterinary  product administration. The highest concentrations (mean values) of amoxicillin after a single administration of the preparation drug tested in chickens were observed after 2 hours. The following pharmacokinetic indices were calculated after application of the studied preparation drug tested in the piglets and slaughter chickens.: T1/2, Cmax, Tmax, AUC, MRT. The performed adaptation of the procedure  of amoxicillin concentrations assay in blood serum of piglets and slaughter chickens confirmed the usefulness of chromatographic methods HPLC for determining the concentration levels in blood. Comparative analysis of the tests results performed with HPLC technique confirmed high effectiveness of the methods used in the research by getting comparable results. The calculated pharmacokinetic parameters for the amoxicillin concentrations in blood serum of piglets and slaughter chickens confirm the differentiation of the rate of elimination depending on the species.The obtained levels of amoxicillin concentrations in piglets after the dosage (28 mg/kg of body weight) and in slaughter chickens after the dosage (20 mg/kg of body weight) let us conclude that pharmacokinetics of amoxicillin give a warranty of effective treatment with the applied doses in spite of differences in species.

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