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Impedimetric RNA assay – detection of the activity of a hairpin ribozyme
|Katarzyna M. Piekielska 1, Sabine Müller 2, Wolfgang Schuhmann 1
1. Ruhr-Universität Bochum, Anal. Chem. - Elektroanalytik & Sensorik (ELAN), Universitätsstr. 150, Bochum 44780, Germany
The hairpin ribozyme belongs to a group of small catalytic RNA structures which is derived from the (-)-strand of the Tobacco ring spot virus satellite RNA. Here, we propose an electrochemical detection system for monitoring of the ribozyme activity using electroche¬mical impedance spectroscopy (EIS). For this purpose, a thiolated RNA substrate is self-assembled at a gold surface. Incubation with the hairpin ribozyme leads to the formation of Watson-Crick base pairs between the ribozyme and its complementary substrate and thus to an assembly of the ribozyme at the RNA substrate monolayer. Cleavage of the substrate which is leading to a disintegration of the ribozyme/substrate complex can be initiated by addition of Mg2+ ions.
Presentation: Short communication at SMCBS'2009 International Workshop, by Katarzyna M. Piekielska
See On-line Journal of SMCBS'2009 International Workshop
Submitted: 2009-08-31 18:07 Revised: 2009-08-31 18:08