Constructing biofuel cells and third-generation biosensors requires direct electron-transfer (DET) reactions, in which the electron transfer occurs directly between enzymes and electrodes.  However, DET has been generally proven for a restricted number of redox enzymes which contain a catalytic domain and additionally a redox domain carrying metallocenters such as heme. One of these multidomain enzymes is cellobiose dehydrogenase (CDH). CDH is an extracellular hemoflavoenzyme, which is secreted by various cellulolytic fungi in the course of cellulose degradation. The CDH carries a flavin and a b-type heme in two separate domains.  The flavin domain of basidiomycete CDH (class I) oxidizes the reducing-end group of cellobiose and higher cellooligosaccharides to their corresponding δ-lactones. The electrons are loaded from the flavin domain to some electrodes via the heme domain. In this study, DET-type bioelectrocatalytic oxidation of CDH from the basidiomycete Phanerochaete chrysosporium using carbon nanoparticles is reported. CDH was adsorbed onto carbon electrodes with carbon black (CB) (PRINTEX^®30, Degussa). CB powder was mixed with DMF (80 : 20, w/w) to prepare a CB slurry. The slurry was applied to a carbon electrode and dried in a drying oven at 60 °C. A CDH solution was then cast on the CB-modified electrode. We have also investigated the optimal conditions of this CDH electrode. The CDH electrode was employed in the third-generation biosensor based and biofuel cell.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/21888 must be provided.

1. Effect of deglycosylation of cellobiose dehydrogenase applied to 3rd generation biosensors and biofuel cells
2. Deglycosylation of glucose oxidase by PNGase F
3. Influence of metal cations on the turnover rate of cellobiose dehydrogenase
4. Electron transfer studies with different sugar oxidizing enzymes and osmium polymers to improve the current density
5. Gold nanoparticle-modified enzyme-based sugar and oxygen sensitive electrodes for biosensing and biofuel cell applications
6. Electrochemical communication between viable bacterial cells and flexible redox polymers
7. Electrochemical Communication between Viable Bacterial Cells and Flexible Redox Polymers
8. Biosensing Applications Of Engineered Pyranose 2-oxidases Wired With Osmium Polymers
9. Anode and cathode reactions for biofuel cells based on direct electron transfer reactions between biological components and electrodes
10. Increasing Biosensor Sensitivity by Length Fractionated Single Walled Carbon Nanotubes
11. Electrical Wiring of Living Bacillus subtilis Cells Using Flexible Osmium-Redox Polymers
12. Some electrochemical properties of laccase immobilised on the Au, IrO_x, or C₆₀-Pd polymer electrode supports
13. Oxygen electroreduction by fungal laccases - combination of electrochemical and spectral data
14. Wiring of whole living bacteria with osmium-redox polymers
15. The electrochemistry of a his-tagged microperoxidase assembled onto gold electrodes
16. Electron Transfer in Complex Two-cofactor-containing Enzymes at Alkanethiol-modified Gold Electrodes