Tissue engineering aims to create functionally active tissue by mixing biomaterials with cells and cell components.Since tissues in vivo are often composed of several types of cell layers, cell/cell interactions are important in the maintenance of a normal physiology in organ systems.To fabricate functional tissue architectures, it is necessary to precisely allocate specific cells to a desired location.Micropatterning of cells is a possible approach for this purpose.One suitable method is to functionalize the surface of the materials with “pro-adhesive” ligands, likely improving subsequent cell adhesion.Our work has concentrated on promoting human endothelial cells(ECs) isolated from human saphenous veins(HSVECs) to adhere to polyethylene terephthalate (PET) substrates.We have grafted REDV peptides onto the surface of a PET template in well-controlled patterns.

For this study,we have used a simple and rapid cell-patterning procedure.Direct-writing techniques,capable of depositing tiny amounts of biological material onto different substrates,are emerging as promising tools for the preparation of miniaturized biosensors due to their versatility,accuracy, low cost and speed.The Laser Induced Forward Transfer (LIFT) technique offers an interesting approach providing a good spatial resolution: tiny amount of material to be deposited was removed from a thin film (inks differing biomolecularly were used) and transferred onto a receptor substrate parallel with the film by the action of a laser pulse.

The main objective is to demonstrate the successful transfer and grafting of REDV peptide spots regardless the solvent used onto prefunctionalized PET by a LIFT technique.The distributions and densities of the peptides were evaluated by high resolution µ-imager using radiolabeled biomolecules.Neither direct nor indirect cytotoxicity to PET-REDV was found to exist regardless the transfer solution used.Finally, surfaces seeded with HSVECs were investigated for cell attachment.

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