PET has been most widely used as biomedical implant because of its desirable properties, such as strength and modulus. Nevertheless, the surface should be improved in order to enhance its biointegration.Some studies have shown that spatial distribution and density of bioactive molecules mainly govern success of biomimetic materials modifications. The aim of this study is to determinate impact of peptide RGD spatial repartition onto cell attachment.

RGD Peptides grafting: The four subsequent steps are: Hydrolyse with NaOH + water/acetonitrile; Oxidation with KMnO₄ in H₂SO₄; NHS and EDC grafting; RGDC grafting.

Micro-patterns creation: Ablation was performed by laser onto the graft surface. Moreover, chemical patterns were formed using conventional photolithographic techniques.

XPS results shown that modified surface exhibits the expected ratios. Grafting ³H-Lysine on PET allow to quantify the density of grafted amino-acid onto the surface and to confirm the stability of the bond after intensive washing and sonicating. Moreover, this tool makes it possible to visualise micro-patterns. Micro-patterns created by laser ablation can also be visualised by optical profilometer.

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1. REDV peptide patterning onto PET using Laser Induced Forward Transfer
2. RGD nanodomains grafting onto titanium surface