A β-estradiol receptor binding mimic was synthesized using molecular imprinting. Polymers (bulk and microgels) were prepared in acetonitrile by using methacrylic acid and ethyleneglycoldimethacrylate as the cross-linker. The selectivity was evaluated by radioligand binding assays. The imprinted polymers were very specific to β-estradiol since the control polymers bound virtually no radioligand. The bulk polymer was employed to screen endocrine disrupting chemicals. Structurally-related steroids like α-estradiol, estrone and ethynylestradiol showed respectively 14, 5 and 0.7 % of cross-reactivities with β-estradiol whereas most unrelated chemicals do not bind at all. These results are compared to those obtained with bioassays using cells in culture bearing β-estradiol-induced genes.

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