Horse-radish peroxidase-modified 3D-hierarchical carbon nanotube electrode - characteristics and potential applications

Leonard Stoica 1Wenzhi Jia 1Chen Jin 2Wei Xia 2Martin Muhler 2Wolfgang Schuhmann 1

1. Ruhr-Universität Bochum, Anal. Chem. - Elektroanalytik & Sensorik (ELAN), Universitätsstr. 150, Bochum 44780, Germany
2. Laboratory of Industrial Chemistry, Ruhr-University Bochum, Universitaetstrasse 150, Bochum 44780, Germany

Abstract

The properties of biosensors or enzyme biofuel cells are unequivocally influenced by intrinsic characteristics of the used biological recognition element, such as the kinetics of the biocatalytic reaction, the presence of activators/inhibitors, etc. However, apart from these primary considerations which might be improved by screening of a large number of specific biological sources or through dedicated genetic modifications, the design of an intimate electronic communication and the robust fixation of redox enzymes at the electrode interface are crucial for benefiting of any particular characteristics of the biological element.
We have followed several directions for studying and designing new bioelectrochemical interfaces. Key parameters such as mass transport, optimum loading with the chosen enzyme, 3D design of the electrode area as well as maintaining efficient electronic conductivity for the entire 3D electrode surface were investigated at modified graphite electrodes [1]. Hence, such materials are considered promising for improving the performances of a bi-enzymatic membrane-less biofuel cell [2].
Recently, new composite materials consisting of double-anchored carbon microfibers (CF) decorated either with carbon-nanotubes (CNT) or carbon-nanoballs demonstrate potential application as biocathode, by their modification with horse-radish peroxidase (HRP), as demonstrated by specific properties for reduction of H2O2, as it was previously observed also for micro-peroxidase [3]. One more time, these experimental observations clearly emphasize the still-not-fully-explored potential by which nanomaterials might contribute to expanding novel properties and characteristics of certain biological elements.
Dedicated applications of the present electrode architecture will be discussed.

References
[1] N. Li, X. Chen, L. Stoica, W. Xia, J. Qian, J. Aßmann, W. Schuhmann, M. Muhler Adv. Mater. 19 2957 (2007).
[2] L. Stoica, N. Dimcheva, Y. Ackermann, K. Karnicka, D. A. Guschin, P.J. Kulesza, J. Ro¬galski, D. Haltrich, R. Ludwig, L. Gorton, W. Schuhmann Fuel Cells 1 53-62 (2009).
[3] T. Lötzbeyer, W. Schuhmann, E. Katz, J. Falter, H.-L. Schmidt J. Electroanal. Chem. 377 291-294 (1994).

 

Related papers
  1. Hierarchical carbon nanotubes composite electrode – towards efficient enzymatic biocathodes based on direct electron transfer
  2. 3D bioarchitectures constructed on carbon cloth for the development of biofuel cells
  3. Electrodeposition of metalloporphyrines for the preparation of electrocatalytically active surfaces
  4. Design of the interface properties of self-assembled thiol/DNA monolayers for improved DNA detection assays.
  5. Impedimetric RNA assay – detection of the activity of a hairpin ribozyme
  6. Scanning electrochemical microscopy into the nanoscale
  7. Laccase-redoxpolymer cathodes for biofuel cells. Evaluation using an electrochemical robotic system.
  8. Pyrolyzed polypyrrole-metal composites immobilized on glassy carbon for gas sensing and catalytic applications
  9. Visualisation of local catalyst activity towards oxygen reduction in hydrochloric acid solution with RC-SECM
  10. Determination of optimum imaging contrast in AC - SECM
  11. Scanning electrochemical microscopy - a tutorial lecture
  12. Electrochemical impedance spectroscopy (EIS) for detection of DNA hybridization in presence of intercalators
  13. Shearforce-based distance control in scanning electrochemical microscopy
  14. Metalloporphyrin modified glassy carbon electrodes for oxygen reduction: Investigation of local electrocatalytic activity
  15. Impact of the Surface Carbonates on the Interaction between CO and ZnO Nanomaterials
  16. Mathematical tools for characterization of spatial variability of surface activity based on SECM images
  17. Visualisation of the effect on catalytic activity of increasing Pt agglomerate sizes using constant-distance mode SECM in a competition mode
  18. Reagentless biosensors based on mediator-modified electrodeposition hydrogels
  19. Microelectrochemistry. From Materials to Biological Applications

Presentation: Short communication at SMCBS'2009 International Workshop, by Leonard Stoica
See On-line Journal of SMCBS'2009 International Workshop

Submitted: 2009-08-31 18:44
Revised:   2009-10-22 10:30