Porous polymeric scaffolds for bone regeneration

Katarzyna Filipczak 3Ireneusz Janik 3Marek Kozicki 3Piotr Ulanski 3Janusz M. Rosiak 3Leonardo Adamo Pajewski 1Radosław M. Olkowski 2Piotr Wozniak 2Anna Chróścicka 2Małgorzata Lewandowska-Szumieł 2

1. Department of Chemistry and Materials, University of L'Aquila, Coppito, L'Aquila 67020, Italy
2. Medical University of Warsaw, Department of Biophysics and Human Physiology, Chałubińskiego 5, Warszawa 02-004, Poland
3. Technical University of Lódź, Institute of Applied Radiation Chemistry (TUL-IARC), Wróblewskiego 15, Łódź 93-590, Poland


One of the aims of tissue engineering is to provide means for self-regeneration of tissues which requires reconstruction due to pathology, trauma or surgery (eg.: tumor resection). Important examples of this approach are devices intended to support regeneration of bones. The design investigated in our project is based on the idea of porous biomaterials - scaffolds - that are strong enough to maintain the mechanical function of the bone segment and in parallel can act as a support for osteoblast proliferation. Depending on the application, degradable or non-degradable materials are desired.

In this work, a technique has been elaborated to synthesize highly porous polymeric scaffolds of controlled pore size, based on non-degradable poly(methyl methacrylate) - PMMA - and degradable poly(ε-caprolactone) - PCL.

It has been found that both kinds of products can be sterilized by ionizing radiation. In order to utilize this possibility in an efficient and controllable way, mechanisms of the underlying radiation-chemical processes have been studied in some detail, using the techniques of pulse radiolysis, laser light scattering, sol-gel analysis, spectrophotometry and viscometry. In case of PCL, irradiation causes an additional effect - cross-linking - that leads to an improvement of the mechanical properties of the scaffold.

In parallel to radiation-chemical studies, first steps towards the target application have been made. Porous, sterile scaffolds of PMMA and PCL were subjected to in vitro studies as supports for cell cultures. It was found that that the products exhibit no cytotoxicity towards fibroblasts and that the osteoblast cells can be efficiently cultured at the surface and in the pores of the scaffolds.

This work has been financed by The Polish State Committee for Scientific Research (grant No 05/PBZ-KBN-082/T08/2002/05) and International Atomic Energy Agency (POL/6/007 and 11513/RO).

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/1808 must be provided.


Related papers
  1. Synthesis of highly biocompatible hydroxyapatite nanopowders
  2. Polyurethane-based material with calcite and aragonite for tissue engineering composite scaffolds
  3.  A  degradable and highly porous polyurethane scaffolds for bone tissue engineering
  4. A bit of physics in our understanding of the cell and tissue interaction with scaffolds
  5. Culture of osteoblasts on nanostructured titanium – preliminary observation
  6. Bone tissue engineered product based on human bone derived cells and polyurethane scaffold
  7. Calcite-based material for tissue engineering ceramic scaffolds
  8. Optimization of polyurethane structure as the potential materials for bone tissue engineering applications
  9. Nanomechanical measurements of thin dextran layers
  10. Cells and scaffolds for tissue engineering - what is needed for success?
  11. Mechanical Properties of Human Plasma Derived Haemostatic Sponges Undergoing Radiation
  12. Human Plasma Proteins as a New Haemostatic Material. Biocompatibility Testing.
  13. Macroscopic and microscopic hydrogels synthesized by radiation technique and their selected biomedical applications
  14. Modification of polymers by ultrasonic treatment in aqueous solution
  15. Effect of diisocyanate chemical structure on biocompatibility of segmented polyurethanes
  16. Poly(epsilon-caprolactone)- and poly(methyl methacrylate)-based scaffolds in human bone-derived cell cultures in vitro
  17. Mechanical characteristics of polymeric support as an important factor in osteoblast culture in vitro
  18. Alginate hydrogel-candidate support for cell transplantation - preliminary observation in human chondrocyte culture
  19. Omentum major as potential source of osteogenic cells for tissue engineering (preliminary report)
  20. Differentiation of cells from adipose tissue into osteoblasts in the presence of biomaterials in vitro (preliminary report)
  21. Alternative methods bring a new input in biocompatibility testing

Presentation: poster at E-MRS Fall Meeting 2004, Symposium B, by Piotr Ulanski
See On-line Journal of E-MRS Fall Meeting 2004

Submitted: 2004-04-28 16:15
Revised:   2009-06-08 12:55
Web science24.com
© 1998-2022 pielaszek research, all rights reserved Powered by the Conference Engine