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Improved initial osteoblast's functions on amino-functionalized titanium surfaces
|Barbara J. Nebe 1, Frank Lüthen 1, Birgit Finke 2, Karsten Schröder 2, Claudia Bergemann 1, Joachim Rychly 1, Rainer Bader 3, Andreas Ohl 2|
1. University of Rostock, Dept. of Internal Medicine, Schillingallee 69, Rostock 18057, Germany
Adhesion and spreading of cells on biomaterials are integrin-mediated processes. But recent findings indicate a key role of the matrix substance hyaluronan (HA) in interface interactions [Zimmermann E et al. 2002]. Because HA is a negatively charged molecule we assume that a biomaterial surface with an opposed charge could boost the cell's first contact to the surface until cells synthesize matrix proteins on its own to mediate the fundamental integrin contact. Polished titanium cp (Ra 0.19µm) was coated with a thin layer of a plasma polymer with amino-groups (Ti PPA). For this purpose, allylamine was polymerized with a microwave excited, pulsed, low pressure, gas-discharge plasma. Collagen was immobilized on Ti PPA with PEG-DA. Human osteoblasts MG-63 were cultured in serum-free DMEM to avoid masking of the coated surface with adsorbed proteins. Adhesion and cell cycle phases were calculated by flow cytometry. Time dependent spreading of cells and stained actin cytoskeleton were measured using confocal microscopy. For quantification of gene expression of osteogenic markers, we used a real-time TaqMan RT-PCR assay.
Presentation: Keynote lecture at E-MRS Fall Meeting 2006, Symposium J, by Barbara J. Nebe
See On-line Journal of E-MRS Fall Meeting 2006
Submitted: 2006-05-26 07:44 Revised: 2009-06-07 00:44