Many are the hypotheses related to the mechanisms of biomineralization process, particularly hydroxyapatite (HA) nucleation and growth. Surface topography is regarded as one of them, as well as supersaturated solutions, the presence of hydroxyl groups, chemistry of the nucleation points, organic components and etc.
The aim of this work is to investigate the influence of the organic components deposited on different solid surfaces upon HA formation. For this purpose the surfaces of stainless steel, silicon and silica glass substrates are coated with extra cellular matrix (ECM) to promote interactions with bone-forming cells. The ECM proteins are known as ligands in reactions with cell surface receptors involved in bone physiology. The osteoblast-like cell line SAOS-2 is allowed to synthesize and assemble its own ECM on the substrates under standard cell culture conditions. Cells are then selectively removed resulting in substrates coated with a thin film of ECM.
The so-modified samples are immersed in a solution, supersaturated with respect to Ca and P ions (so called simulated body fluid, which resembles the composition of human blood plasma) and a layer is grown. X-ray diffractometric techniques (XRD and EDX), spectroscopic (FTIR and Raman), microscopic techniques (SEM) are used to highlight the likeness of the grown artificial biomimetic HA/ECM protein structure with natural bone tissue.
A homogeneous HA layer is found on the so-modified samples, which consists of particles with regular sphere shape with mean diameter size of 5 micrometers. Well resolved IR and Raman peaks reveal the formation of crystalline HA layer, self organized inside the ECM matrix.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/655 must be provided.

1. Synthesis of Micro- and Nano-Sized LiNi_0.5Mn_1.5O₄ and LiNi_0.5Mn_0.5O₂ Materials and Studies of Their Electrode Behavior in Li-Ion Cells
2. Patterning of Surfaces by Polysilicon for Inducing Hydroxyapatite Growth by Laser-Liquid-Solid Interaction
3. Natural opal as a model system for studying the process of biomineralization
4. Hydroxyapatite growth on surfaces modified by CdSe or Si nanoparticles embedded in SiOx thin films
5. Growth of hydroxyapatite layers on modified by ion beam patterned solid surfaces for studying the biomineralization