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Evaluation of bioceramic bone substitutes - hydroxyapatite (HAP), tricalcium phosphate (TCP) and biphasic ceramic (HAP/TCP) in vivo

Ilze Salma 1Mara Pilmane 1Girts Salms 1Janis Locs 2Liga Berzina-Cimdina 2

1. Riga Stradins University (RSU), Dzirciema 16, Riga LV1007, Latvia
2. Rudolfs Cimdins Riga Biomaterials Innovation and Development Centre of Riga Technical University (RCRBIDC), Pulka street 3/3, Riga 1007, Latvia


In clinical practice different bone substitute biomaterials are used for maxillary sinus floor augmentation. Due to biocompatibility with living body and bioactivityhydroxyapatite (HAP), tricalcium phosphate (TCP) and biphasic ceramic (HAP/TCP) are frequently used and are the most promising due to the particular biodegradation.  Datain the literature of tissueresponses to the bioceramic materials are different and sometimes controversial.The aim of our study was to evaluate bone and soft tissue response in animal experiments.

CaP powders used for bone substitutes preparation was synthesized by wet precipitation reaction between calcium hydroxide and phosphoric acid. The green bodies – granules in size range from 0.5 to 1 mm were formed and sintered to obtain HAP, TCP or HAP/TCP ceramics.

Intraosal implantation of bioceramic granules was performed in rabbit jaw. Bone and soft tissue with biomaterial was obtained after three months. Tissues were proceeded for the detection of growth factors – bone morphogenic protein (BMP2/4) and transforming growth factor (TGFβ), proinflammatory cytokines and using TUNEL method – apoptosis.

Results demonstrated most pronouncedproinflammatory cytokines expression after implantation of TCP bioceramic. Results after biphasic ceramic implantation showed less cell apoptosis.

Conclusions. Ourresults demonstrated higher biocompatibility for biphasic bioceramic HAP/TCP (60/40) compared with HAP and TCP.



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Presentation: Oral at Nano and Advanced Materials Workshop and Fair, by Ilze Salma
See On-line Journal of Nano and Advanced Materials Workshop and Fair

Submitted: 2013-06-02 14:40
Revised:   2013-06-02 14:40