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Droplet based lab-on-chip microfluidic microsystems for high sensitive mass spectrometry analysis
|Florian Lapierre 1,2, Gaëlle Piret , Guillaume Perry , Vincent Thomy , Yannick Coffinier , Rabah Boukherroub 1,2|
1. Institut de Recherche interdisciplinaire (IRI), 50 avenue de Halley, BP 70478, Villeneuve d'Ascq 59652, France
Since the emergence of Lab-On-Chip applications for biomedical protocols, electrowetting on dielectric-digital microfluidic (EWOD-DMF) systems have been implemented in many domains such as enzyme assays, immunoassays, DNA-based applications, cell-based assays, tissue engineering and proteomics [1,2]. Thus, Digital MicroFluidic (DMF) Lab-On-Chip devices have been coupled with off-line Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) analysis as an alternative to optical methods . MALDI-MS analysis brings the advantage of time reduction, less complexity of sample preparation and high sensitivity. Even though this system is a pioneering one in the LoC applications for MS analysis, several drawbacks can be underlined: proteins or peptides concentrations remain still quite high (> nmol/µL) and the analysis protocol is somewhat time consuming and complicated due to manipulation of viscous matrix and to proteins drying on the surface.
In this presentation, I will discuss an original study consisting of coupling EWOD-DMF and matrix-free Laser Desorption/Ionization Mass Spectrometry (LDI-MS) analysis using superhydrophobic nanostructured silicon substrates. Compared to a classical hydrophobic surface, it leads to an improvement of the microfluidic actuation (both in terms of minimal applied voltage threshold and droplet speed) and it allows a matrix free LDI-MS analysis of very low concentration of peptides sample (down to fm/µL) through a rapid and simple protocol (without addition of any organic matrix) [3-6].
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Presentation: Keynote lecture at SMCBS'2011 International Workshop, by Rabah Boukherroub
See On-line Journal of SMCBS'2011 International Workshop
Submitted: 2011-08-08 14:24 Revised: 2011-08-08 14:27