At iNANO we aim at the development of new electrochemical biosensor technologies for analysis of cancer gene biomarkers, pathogens and small molecules of interest, which is crucial for efficient diagnostics of cancer and infectious diseases, and neurological disorders. We use small, functional DNA and RNA nanoblocks to create molecular assemblies with improved structural and biosensing properties that in combination with electrochemistry approach may provide extremely sensitive and accurate, yet simple, inexpensive and robust gene-based sensing platforms. I will overview the design and our studies of electrochemically-labeled DNA/RNA beacon structures that undergo distinct structural changes at electrodes upon hybridization phenomena (i.e. chemical recognition of one DNA sequence by another) or upon binding of other molecules of interest. In the latter case, we use so-called aptamers, which are nucleic acids designed to selectively bind to certain analyte, from small molecules to proteins and cells. I will discuss the general limits for selectivity and sensitivity of the developed assays.

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1. Electrochemistry of redox-labelled DNAs immobilized onto electrodes through the de-novo designed triazene linker 
2. Restructuring and cleaavage of ferrocene-alkanethiol ester SAMs in lipase-based activity assays.
3. Electrochemical DNA biosensors for label-free detection of nucleic acids exploiting DNA conformational transitions upon hybridization event
4. Electron Transfer Reactions of Heme-containing Enzymes at the Electrodes: Bioelectrocatalysis and its Optimisation
5. Electron Transfer in Complex Two-cofactor-containing Enzymes at Alkanethiol-modified Gold Electrodes