Introduction: Nano-scaffolds have provided good conditions for cell adhesion and are currently being applied to many different types of implantable medical device. The authors have produced and evaluated nanofibers made from biocompatible and biodegradable polymers for scaffold via electrospinning method. In general, when nanofiber non-woven mats are used as scaffold, cells would not enter into a scaffold because of its high bulk density in the direction of the thickness. In this study, authors have contrived a convenient method to make spongiform nanofiber 3-dimensional fabric, which has spaces in moderation for scaffold.  The property and tissue compatibility of nanofiber fabric made by this method was investigated. Materials and Methods: PGA (Sigma-Aldrich, Inc., USA) was dissolved in 1,1,1,3,3,3 hexafluoro-2-propanol at concentration of 67 mg/ml. Collagen (Nippon Meat Packers, Inc., Japan) was dissolved HFIP at concentration of 100 mg/ml. The mixture at 5:5 solution was electrospunned at 3 ml/hr. The positive output lead of a high voltage supply, set to 30 kV, was attached to a 25 gauge needle on the syringe. Spinning stainless bath filled with t-butyl alcohol was used as a grounded target. A grounded target was placed 30 cm from the needle tip. After making nanofibers in the spinning stainless bath via electrospinning method, nanofiber fabric was vacuum-freeze dried using a t-BuOH freeze dryer in 1 day. Specimens were punched out from the nanofiber fabric. The fiber diameter and structure of nanofiber fabric were observed by scanning electron microscope (SEM, JSM-5600LV, JEOL Ltd., Japan). Results and Discussion:  The nanofiber fabric consisted of fibers with diameters ranging from 140 nm to 1160 nm. Most of the fiber diameter are less than 1mm, and the average diameter is 760 nm (± 290 nm). The nanofiber fabric had a spongy structure with large spaces between the nanofibers. The tissue ingrowth in the spongiform nanofiber fabric was observed in vivo.

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