In this study, a scaffold was designed for use in bone tissue repair and the effect of glutaraldehyde (GA) concentration as crosslinking agent was investigated. To mimic the mineral and organic component of natural bone, hydroxapatite (HA) and gelatin (GEL) were used as the main components of this composite. Nano-powders of HA were synthesized, and together with GEL, was used to engineer a three-dimensional nanocomposite with combination of three techniques of layer solvent casting, freeze-drying and lamination. The synthesized nanocrystalline hydroxyapatite and nanocomposite samples were characterized by the commonly used techniques. The results show that GEL/HA nanocomposite is porous with 3-dimension interconnected structure, pore sizes ranging from 300 to 500 µm, and about 85% porosity. In addition, increasing GA concentration provokes enhancement of compressive strength and reduction of toughness. It was concluded that optimum concentration for cross-linking GEL matrix for this purpose is 1%. Cultured osteoblast-like cells (SaOS-2) have shown an excellent level of cytocompatibility for nanocomposite scaffold. A specific cobmination of commonly used techniques was applied to engineer a scaffold with almost ideal properties intended for bone tissue engineering is introduced. In addition, scaffolds that are prepared via this compound process has the potential to be used in solid free form applications and so being formed in any dimention and geometry relevant to defect size and shape.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/15628 must be provided.

1. Preparation and rheological properties of calcium phosphate-based paste used for treatment of vesicoureteral reflux