The arrays of artificial receptors formed by N-lipidated oligopeptides immobilized, in the highly ordered fashion, on the solid support were synthesized and used in the studies on molecular recognition. Thus, even in the case, when the single receptor in a differential array does not necessarily have selectivity for a particular analyte [1], the combined fingerprint response can be extracted as a diagnostic pattern visually, or using any chemometric tools.

The previous studies confirmed that alternation of the binding pattern could detect even tiny structural changes of guest molecules and therefore offer a new tool for SAR studies. An assay of physiological fluids and tissue homogenates has been found useful for diagnosis of thyroid tumors [2].

Further successful application of arrays of artificial receptors for profiling of metabolome depends on efficiency of competitive binding of guest molecules and colored indicator dye. Therefore we attempted to measure the rate of binding of coloured ligands in the search for the most efficient reported dye.

The rate measurements were performed by spectrophotometric method using 96 wells ELISA plate reader.

Acknowledgements: The study was supported by the Polish State Committee for Scientific Research under the Project 2 P05F 03130.

[1] Wright A.T.; Anslyn, E.V. Chem. Soc. Rev., 2006, 35(1), 14-28.
[2] Balcerzak, W.; Bednarz, W.; Domoslawski, P.; Olewinski, R.; Kolesinska, J.; Kaminski, Z.J.; Dziarkowska, K.; Wieczorek, P. Polish J. Endocrin. 2006, 57(4), 308-313.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/14781 must be provided.

1. Preliminary studies on application of library of artificial receptors for differentiation of metabolites in urine of healthy and cancer mice
2. Synthesis of chiral triazine coupling reagents based on esters of N-alkylproline and their application in the enantioselective incorporation D or L amino acid residue directly from racemic substrate
3. 1,3-Oxazolidin-5-ones as chiral components in the synthesis of traceless enantioselective reagents
4. Attempts to application of libraries of N-lipidated amino acids and peptides immobilized   on cellulose as a stereosectors
5. An Approach for Application of Synthetic Peptides as Markers of Atherosclerotic Diseases
6. Synthesis and Antimicrobial Activity of P-Tria- zinylphosphonium Salts
7. Traceless chiral coupling reagent. A new concept for synthesis of optically active products from racemic carboxylic acids.
8. An approach towards efficient peptide synthesis in aqueous solution
9. Synthesis and application of library of artificial receptors immobilized on cellulose support
10. Design and synthesis of libraries of artificial enzymes
11. Sulfonates of N-Triazinylammonium Salts as Highly Efficient and Environmentally Friendly Triazine-Based Coupling Reagents
12. Synthesis of Building Blocks Containing Glycosyl Moieties by Using Triazinylammonium Sulfonates
13. Transformation of Tertiary Amines into N-Triazinylated Analogues by Treatment with Chloro-1,3,5-Triazines
14. The New Analogues of Nitrogen Mustard With One, Two or Three 2-Chloroethylamino Fragments. Reactions with Nucleophiles
15. Synthesis and Screening of the Library of the Supramolecular Structures Formed by N-Lipidated Oligopeptides for Selection of Host Active as Artificial Esterase
16. AN APPLICATION OF THE ARRAY OF LIPIDATED OLIGOPEPTIDES RESEMBLING ARTIFICIAL RECEPTORS FOR MOLECULAR RECOGNITION. THE STUDIES ON THE LIPOPHLILICITY OF SOME PIPERAZINES
17. SYNTHESIS AND SEROLOGICAL INTERACTIONS OF H.PYLORI UREASE FRAGMENT 321-339 IMMOBILIZED ON THE CELLULOSE SUPPORT
18. AN APPLICATION OF CHIRAL COUPLING REAGENTS FOR ENANTIOSELECTIVE ACTIVATION OF RACEMIC N-BENZOYL- 2-HYDROXYMETHYL-2-AMINO ACIDS
19. THE NEW WATER SOLUBLE SALTS, PRONE TO SPONTANEOUS DECOMPOSITION WITH FORMATION OF 2-CHLOROETHYLAMINO MOIETY (NITROGEN MUSTARD DERIVATIVES)