Immobilization of chemical species onto solid substrates is one of the hottest topics of contemporary surface chemistry. We present two complementary approaches towards molecule immobilization - through coordination to self-assembled monomolecular films or encapsulation within polymeric hollow structures. Monomolecular layers provide an opportunity to define their chemical functionality with molecular precision that can lead - via the appropriate synthetic route - to covalent or electrostatic attachment of desired molecules to the surface. The strategy for molecule immobilization (pyrene, perylene or laccase from Cerrena unicolor) reported here relies on ionic coordination of –COO^- terminal groups (present on the immobilized molecule) to zirconated phosphate (ZP) monolayers. As an alternative to this approach we developed a novel method of immobilization through formation of polymeric hollow structures that are capable to entrap the molecules inside the hollow: the polymeric material is deposited onto the liquid droplet containing dissolved chemical species. We used several experimental techniques to confirm that both approaches allow for stable immobilization. The immobilized molecules can be subsequently used as elements of sensor or optical devices, as well as electrodes in fuel electrochemical cells.

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