Soy-based infant formulas are used as alternative source of nutrients in cases of milk protein intolerance, food allergy, celiac disease and malabsorption. These formulas are composed of soy protein isolate, soy or coconut oil and mineral components.

The aim of this research is to determine the immunogenic properties of protein and peptide fractions isolated from the selected soy-based infant formulas – Prosobee, Isomil and Bebilon. The cross-reactivity between the isolated proteins and the bovine casein, soy 7S globulin and wheat albumin and globulin has been studied.

Albumins, globulins and peptides were isolated from the investigated material and analyzed by HPLC and SDS-PAGE. PAS staining of the electrophoretic gel has shown that all analyzed proteins were glycoproteins. Polyclonal antibodies were used for ELISA and Western-immunoblotting of peptides and proteins. The influence of extracts on peristalsis in rabbit isolated ileum has been evaluated.

Western-immunoblotting has shown the cross-reactivity of soy-based infant formulas with both soy and wheat proteins but not with bovine casein. The presence of the mean soy allergen fraction - 7S globulin has not been shown on SDS-PAGE. Though antibodies raised against protein extract of those formulas fully recognize purified soy 7S globulin. Probably soy-based infant formulas contain fragments similar to soy 7S globulin.

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1. Determination of µ-opioid receptor antagonistic peptides in selected dairy products by enzyme-linked immunosorbent assay (ELISA)
2. Influence of glycation (non-enzymatic glycosylation) on antigenicity of soy (Glycine max) and pea (Pisum sativum) 7S globulin
3. Human β‑casomorphin transport across Caco-2 monolayer