<>It has been observed that glucose is essential for ferricyanide reduction in erythrocytes; however ferricyanide reduction does not lead to a significant increase in the rate of glucose consumption.
We checked the influence of glucose starvation (four-hour incubation with deoxyglucose) on ferricyanide reduction in the wild-type strain of S. cerevisiae cells. Ferricyanide was added to a final concentration of 0.1 mM or 1 mM. The product of the reduction, ferrocyanide, was estimated with 1,10–phenanthroline as reported by Avron and Shavit. Reduction of ferricyanide by the yeast in MES buffer (10 mM, pH 6,5) only or in MES buffer with deoxyglucose was about 80% lower than in YPD medium. Interestingly the same low level of reduction was observed when cells were incubated in the buffer with glucose suplemented to a final concentration 2% for four hours. The major conclusion from this study is that the stimulation of substrate reduction stimulation correlated with the concentration of glucose during incubation (cells were kept in MES buffer for 1, 2, 3 and 4 hours and then ferricyanide and glucose were added). Under these conditions the reduction was maintained at the same level as in the control. The level of ATP was determined using the luminometric method and was not significantly correlated with the capacity for ferricyanide reduction.

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