Electrochemically synthesized molecularly imprinted polymer for determination adenosine 5'-triphosphate (ATP)

Tan-Phat Huynh 1Agnieszka Pietrzyk-Le 1Chandra Bikram KC 3Krzysztof Noworyta 1Francis D'Souza 3Wlodzimierz Kutner 1,2

1. Polish Academy of Sciences, Institute of Physical Chemistry, Kasprzaka 44/52, Warszawa 01-224, Poland
2. Cardinal Stefan Wyszynski University in Warsaw, School of Sciences, Faculty of Mathematics and Natural Sciences, Dewajtis 5, Warsaw 01-815, Poland
3. University of North Texas Denton, Department of Chemistry, 1155 Union Circle 305070 TX, Denton 76203, United States

Abstract

Sensitive to the adenosine 5’-triphosphate (ATP) nucleotide tailor-made molecular cavities were imprinted in a thiophene polymer network to devise and fabricate a novel chemical sensor for determination of ATP. A molecularly imprinted polymer (MIP) as a selective recognition material of the chemosensor was prepared by potentiodynamic electropolymerization. Three different functional monomers were used for complexation of one ATP molecule. The uracil substituent of bis(2,2’‑bithien‑5‑yl)methane complementary paired the adenine moiety of the target nucleotide analyte. Besides, the boronic acid of reversibly bound vicinal diol of the ribofuranose moiety. Three phosphate groups of ATP in their anionic forms, were bound with hydrogen bonds to the amide moiety of bis(2,2’‑bithien‑5‑yl)methane. In order to probe the effect of different binding motifs adopted in the present ATP recognition and to visualize the structure of the supramolecular complex formed, DFT calculations at the B3LYP/6-31G(d) level were performed. The signal transducer of this chemosensor was a 10 MHz quartz crystal resonator of an electrochemical quartz crystal microbalance (EQCM) and a Pt disk electrode for the piezoelectric microgravimetry and capacitance determination, respectively, under flow-injection analysis conditions. Properties of the MIP film were unravelled by X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy.

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Presentation: Poster at SMCBS'2011 International Workshop, by Tan-Phat Huynh
See On-line Journal of SMCBS'2011 International Workshop

Submitted: 2011-10-21 17:36
Revised:   2011-10-21 17:45
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