We present a universal biosensing platform for virus or antibody label-free recognition. It was produced using electrodeposition of silver nanoparticles onto tin-doped indium oxide (ITO) by cyclic voltammetry. Our method is consistent with the principles of green chemistry. Morphology of substrates was changed with tuning the scan rate of the applied potential and the number of scans. The platform is suitable both for surface enhanced Raman spectroscopy (SERS) and localized surface plasmon resonance (LSPR). The obtained substrates are reproducible and stable. Efficacy of our AgNPs substrate for biosensing was demonstrated for the detection of neurotransmitters such as choline at low concentration and with short detection time at SERS and for detection of specific binding between avidine and biotinylated bacteriophages at both SERS and LSPR.

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