Droplet based lab-on-chip microfluidic microsystems for high sensitive mass spectrometry analysis

Florian Lapierre 1,2Gaëlle Piret Guillaume Perry Vincent Thomy Yannick Coffinier Rabah Boukherroub 1,2

1. Institut de Recherche interdisciplinaire (IRI), 50 avenue de Halley, BP 70478, Villeneuve d'Ascq 59652, France
2. Institut d'Electronique de Microelectronique et de Nanotechnologie, UMR CNRS 8520 (IEMN), BP 60069 - Cité scientifique-avenue Poincaré, Villeneuve d'Ascq 59652 Cedex, France

Abstract

Since the emergence of Lab-On-Chip applications for biomedical protocols, electrowetting on dielectric-digital microfluidic (EWOD-DMF) systems have been implemented in many domains such as enzyme assays, immunoassays, DNA-based applications, cell-based assays, tissue engineering and proteomics [1,2]. Thus, Digital MicroFluidic (DMF) Lab-On-Chip devices have been coupled with off-line Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) analysis as an alternative to optical methods [3]. MALDI-MS analysis brings the advantage of time reduction, less complexity of sample preparation and high sensitivity. Even though this system is a pioneering one in the LoC applications for MS analysis, several drawbacks can be underlined: proteins or peptides concentrations remain still quite high (> nmol/µL) and the analysis protocol is somewhat time consuming and complicated due to manipulation of viscous matrix and to proteins drying on the surface.

In this presentation, I will discuss an original study consisting of coupling EWOD-DMF and matrix-free Laser Desorption/Ionization Mass Spectrometry (LDI-MS) analysis using superhydrophobic nanostructured silicon substrates. Compared to a classical hydrophobic surface, it leads to an improvement of the microfluidic actuation (both in terms of minimal applied voltage threshold and droplet speed) and it allows a matrix free LDI-MS analysis of very low concentration of peptides sample (down to fm/µL) through a rapid and simple protocol (without addition of any organic matrix) [3-6].

 

[1] L. Malic, D. Brassard, T. Veres and M. Tabrizian, Lab Chip 10 (2010) 418-431.

[2] V. Srinivasan, V. Pamula, and R. Fair, Lab Chip 4 (2004) 310-315.

[3] A. R. Wheeler, H. Moon, C. A. Bird, R. R. O. Loo, C. J. Kim, J. A. Loo, R. L. Garrell, Anal. Chem. 77 (2005) 534

[4] F. Lapierre, G. Piret, H. Drobecq, O. Melnyk, Y. Coffinier, V. Thomy and R. Boukherroub, Lab Chip11 (2011) 1620-1628.

[5] M. Jönsson-Niedziolka, F. Lapierre, Y. Coffinier, S. J. Parry, F. Zoueshtiagh, T. Foat, V.

      Thomy, R. Boukherroub, Lab-on-Chip 11 (2011) 490-496

[6] G. Perry, V. Thomy, M. R. Das, Y. Coffinier and R. Boukherroub (2011)

 

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Presentation: Keynote lecture at SMCBS'2011 International Workshop, by Rabah Boukherroub
See On-line Journal of SMCBS'2011 International Workshop

Submitted: 2011-08-08 14:24
Revised:   2011-08-08 14:27