Colloidal magnetic nanoparticles have found effective applications in drug and gene delivery, magnetic resonance imaging (MRI), tissue engineering, and hyperthermic therapies for past decade. In these respect, 3D suspensions of magnetic nanoparticles with various functional surface layers were produced and tested in-vitro and in-vivo. Unusually strong magnetic properties and biocompatibility are considered to be one of the important issues for engineering of new materials for biomedical applications. In this research the surface of superparamagnetic iron oxide nanoparticles (SPION) was functionalized with two biocompatible materials such as gold and L-aspartic acid to study their reaction with human fibroblasts, thus providing information about cytotoxicity, endocytosis and particles adhesion to cells. Au-coated SPION sample was prepared via process based on the reduction of corresponding metal ion on the surface of particles. The chemisorption process was used to modify surface of SPION with L-aspartic acid. Surface functionalized SPION samples were incubated with human fibroblasts for 6 and 24 hr. Cell-particles interaction were observed by light, fluorescence and scanning electron microscopy. Cells incubated with L-aspartic acid-coated SPION have similar gross cell morphologies to control cells, indicating no adverse cell reaction to the presence of the particles. With regard to the cell cytoskeleton, prominent stress fibre formation and tubulin clearly radiating out from the tubulin organising centre by the nucleus was observed, again exactly as the control cells. L-aspartic acid-coated SPION sample demonstrated an increase in clathrin production, particularly at the cell filopodia, suggesting that the particles are partially being up-taken into the cell body via endocytosis; however this is not causing any cell damage after 24 hours culture. Au-coated SPION sample do affect cell size and appear to induce smaller filopodia with cell/particle debris.

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