Ti and Al alloys are extensively employed thanks to their excellent biocompatibility, corrosion resistance, mechanical properties and workability. Better performance and workability can be achieved through new compositions and/or modifications of the available Ti alloys. The deposition of nanocrystalline hydroxyapatite by a method previously described (Bigi et. Al 2005) onto nanostructured Ti13Nb13Zr substrates and standard Ti6Al4V substrates promote cells proliferation and adhesion. In order to investigate the potential application of these alloy substrates in tissue engineering , this study was conducted with the aim of using this materials for in vitro proliferation of human bone-marrow-derived mesenchymal progenitors (bmMSC). Coated and uncoated substrates were submitted to structural and morphological characterization. In order to determine the proliferation rate of cells cultured on biomaterials, the MTT test was performed. These in vitro studies showed that MSC proliferation progressively increases from 7 to 28 days of cultures showing lower MTT values of Al alloys vs Zr one. Molecular biology analyses for the principal bone markers, osteocalcin, osteopontin, collagen type I, confirm the correct commitment of the bmMSC in presence of coating of nanocrystalline hydroxyapatite. In particolar Zr alloys with coating of nanocrystalline hydroxyapatite show higher expressions. Morphological analyses performed by means of SEM analyses, showed that on the nanostructured surface of the alloys cells attached and spead well.This ultrastructural analysis supported evidence that MSC grown onto Zr alloy with coating of nanocrystalline hydroxyapatite show a phenotype osteocyte like.

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