In a process of receiving new chemical compounds applied to biological tests purification is essential. There is variety of efficient purification methods in organic chemistry such as: precipitation, evaporation, extraction or chromatographic methods like Flash, TLC, and HPLC. In the case of library of small samples -quantity of milligrams - classic methods can not be applied easily.

Usefulness of reversed-phase gradient HPLC method to isolation of PPAR libraries is shown in this publication. Fast and efficient separation of several compounds with very good yield and purity has been achieved. Duration of analysis is about 20 minutes (2 injection per sample). Identities of isolated substances have been verified by HPLC/MS analysis. The molecular ions of mean analytes and theoretical mass calculated from chemical equation have been compared. Purity of isolated compounds was 80 - 100 % depending on the initial contamination of the sample. Quantity of isolated substance was from 1 mg to 100 mg .

Direct scaling from analytical to semi-preparative HPLC has been done. This allows predicting retention times in the preparative system just by analyzing sample in the analytical system. Limitation of preparative columns has been noticed - larger particles size causes worse resolution.

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1. New phenylpropanoic acid derivative with antidiabetic potential acting as a partial PPAR gamma agonist – preclinical studies.
2. New phenylpropanoic acid derivative with antidiabetic potential acting as a partial PPAR gamma agonist – preclinical studies.
3. SYNTHESIS OF NEW 3-PHENYLPROPIONIC ACID DERIVATIVES HAVING ANTIDIABETIC ACTIVITY