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Enzymic catalysis in supercritical carbon dioxide
|Marie Zarevúcka 1, José P. Coelho 2, Jean S. Condoret 3, Roumiana P. Stateva 4|
1. Czech Academy of Sciences, Institute of Organic Chemistry and Biochemistry, Flemingovo n. 2, Prague 16610, Czech Republic
The use of biocatalysts in SC-CO2 has received widespread attention in recent years because of many advantages. Biocatalysts demonstrate substrate specificity under mild reaction conditions and SC-CO2 has several advantages over liquid solvents such as high solute diffusivities and low viscosity, which accelerate mass transfer-limited enzymatic reactions.
Two types of enzymes (lipase and glucose 2-oxidase from Ganoderma lucidium) will be tested under SC-CO2 from the point of view of enzyme stability and enzyme specificity. The effects of molar ratio of the substrates, water content, enzyme load, pressure and temperature, time course and substrate flow rate on both the rate of enzyme reaction and product distributions will be estimated.
The kinetic, thermodynamic and transport phenomena have a pronounced influence on enzyme catalysed reactions, and their understanding is a key step in the modelling and design of novel processes which operate at high pressures and involve phase and chemical equilibrium. Thus, particular attention will be given to studying and modeling the kinetics of the enzyme-catalysed reactions, to modeling the simultaneous phase and chemical equilibrium.
Acknowledgement: Czech part of the project (D30.001) founded by the Ministry of Education of the CR..
Presentation: Oral at COST action D30 Mid term evaluation meeting, by Marie Zarevúcka
See On-line Journal of COST action D30 Mid term evaluation meeting
Submitted: 2006-01-03 12:04 Revised: 2009-06-07 00:44