Amperometric biosensors with H₂O₂ detection are important and widely used analytical systems. In case of long lifetime and stability of immobilised biolayers, the electrode system becomes the limiting factor for the long-term biosensor use. This is associated with the working electrode surface passivation and thus decreased real polarisation voltage on the active surface. In this study, a chemically modified transducer with poly(m-phenylenediamine, resorcinol) / Prussian Blue / Pt electrode was prepared via electrodeposition of Prussian Blue as electron transducer followed by electropolymerisation coating by a selective diffusion layer of m-phenylenediamine and resorcinol. This system was compared with the bare Pt electrode. Both systems were designed as glucose biosensors by immobilisation of glucose oxidase as a top layer of the sensor. Ag/AgCl was used as a reference electrode. The long-term stability and response in dependence on the polarisation voltage was studied. The influence of interferents was also evaluated. The possibility of extending this system into multichannel configuration was tested and suitable methods of enzyme layer were developed and compared.

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