Pharmaceutical preparations containing corticosteroid salts and esters such as betamethasone and its related compounds have been recently applied in cream, injections and lotions in dermatological [1]. The official method for identification and assay of betamethasone derivatives in the presence of other ingredients including antibiotics and preservatives is high performance liquid chromatography (HPLC) [2,3]. This method is highly efficient for the determination of betamethasone but very tedious and time consuming because it requires repeated solvent extraction of multicomponent pharmaceutical preparations. Therefore we tried to develop a rapid and inexpensive TLC-densitometric method for separation and identification of betamethasone and some betamethasone derivatives (salts and esters). To achieve satisfactory separation of examined betamethasone and betamethasone compounds various chromatographic conditions (different adsorbents and mobile phases) in normal and reversed phase system were applied (NP-TLC and RP-TLC).

The results of this study might be an inspiration for further investigations concerning the quantitative analysis of examined betamethasone compounds.

1. Pastuszka, M.; Kaszuba, A. Status of combination drugs with betamethasone dipropionate and salicylic acid in treatment of skin diseases. Postęp. Dermatol. Alergolog., XXIX, 2012/13, 196-204.

2.  Polish Pharmacopoeia, Polish Pharmaceutical Society, Warsaw, Poland, 2011.

3. The United States Pharmacopoeia, Twinbrook Parkway, Rockville, Md, USA, 2011.

Acknowledgement:

This research was financed by the Medical University of Silesia as part of statutory research project in 2013 year, project No. KNW-1-013/N/3/0.

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1. Evaluation of the lipophilic properties of betamethasone and its related compounds
2. Microarray analysis of CYP’s gene expression in human dermal fibroblasts with cyclosporine A