Particle size distribution in lactose solution varied from 0.9 to 5 nm in diameter. Analysis of the results showed that, with increasing lactose concentration of a solution, the modes of the curves were displaced in the direction of increasing particle size (Fig. 1B). This finding accords with the theory that, in saturated solutions, molecules rapidly form aggregates. With decreasing of temperature the modes of the curves at all concentrations showed a displacement on the scale towards increasing particle size (Fig. 1A). This is due to the fact that a decreasing temperature promotes the growth of aggregates.

Fig.1 A - effect of temperature on particle size distribution in lactose solution of 40% w/v; B – effect of concentration of lactose solutions on particle size distribution at 30 °C

The results presented in this work indicate that the measurement of temperature and the concentration effect on particle size distribution in a lactose solution is now possible using the dynamic light scattering technique.

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