It has been reported that a modification to the vapor diffusion technique, in which desiccant was used instead of reservoir solution to absorb the solvent evaporated from the protein crystallization droplets, increased the protein crystallization screening success rate. However, because the optimal amount of desiccant for a specific crystallization attempt is unknown, this desiccation method (one-time desiccation method) is not easy to apply broadly.

To solve this problem, we propose a further modification to the one-time desiccation method. We perform multiple desiccations (i.e., repeat the desiccation procedure more than once) until the droplets are dried out, so that the crystallization droplets can achieve a large concentration range. Compared with the one-time desiccation method, the gradual desiccation method (GDM) showed a higher crystallization screening success rate. In this report, the experimental results of crystallization using the GDM were discussed.

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