Background: Accumulating studies show that excessive F exposure causes a variety of pathological changes in various tissues including the heart. In this study, we investigated the effect of sodium fluoride (NaF) on cardiac function in a murine heart with a pressure-volume conductance system and an ex vivo heart perfusion system.

Methods and Results: Adult male C57BL/6 mice were administrated with NaF 30 mg/mL, 70 mg/mL, and 150 mg/mL (corresponding to 2.84±0.29, 6.28±0.61, and 14.18±1.00 mg F/g body weight per day) by gavage for 4 weeks. The pressure-volume loop data showed that NaF treatment for 4 weeks significantly modulates the heart rate (HR) and cardiac output (CO) of a mouse heart in a dose-dependent manner. A low dose (30 mg/mL) of fluoride did not alter cardiac function, while a high dose (70 mg/mL and 150 mg/mL) of fluoride reduced HR and CO of mouse hear (n=4-6 per group, p<0.05 vs. vehicle control). To further address whether NaF alter the left ventricular contractile function, isolated mouse hearts from these groups were subjected to 20 min global ischemia followed by 30 min reperfusion in an ex vivo perfusion system. Intriguingly, the results demonstrated that post-ischemic left ventricular function was improved by NaF treatment (150 mg/mL) when compared to vehicle control group (135 ± 5% vs. 75 ± 0.7% of baseline, p<0.05 vs. control group) However, a medium dose of NaF (70 mg/mL) significantly decreased post-ischemic left ventricular contractile function of mouse heart (23 ± 0.4% vs. 75 ± 0.7% of baseline, p<0.05 vs. control group) and no changes in low dose group.

Conclusions: Taken together, this data suggests that fluoride reduced contractile function of the heart in response to ischemic stress. This data also warrants further investigation regarding the effects of fluoride on cardiovascular health.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/28320 must be provided.

1. Effect of Fluoride on the Apoptosis and Expression of Casepase-3 and Caspase-9 in Rat Osteoblasts