Indicators of fluoride accumulation are ones of the extremely sensitive and very important parameters of changes in the environment. Fluoride environmental pollution is thus in modern civilization, one of the major environmental problems due to the toxic effects of this element for plants, animals and humans. Therefore it is important to continuously monitor the content of fluorine compounds in the ecosystems. Monitoring of fluoride environmental pollution should include sensitive species and changes in biochemical, physiological, histological and genetic properties.
The aim of this study was to determine in the soil the concentrations of fluoride which cause 50% root growth inhibition of two plant species: white mustard (Sinapis alba L.) and spring wheat (Triticum aestivum L.). The study was conducted in 2011 in the laboratory of Department of Biochemistry , West University of Technology in Szczecin. Soil samples characterized by granulometric composition of light clay and 1.10% organic carbon were collected from the black-earth of Gumieniecka Plain
The phytotoxicity test consisted of two cycles: a preliminary test and final test. Preliminary studies were performed to determine the concentration range of compounds that affect the quality of the soil. Therefore, according to ISO-11269-2:1995 method, NaF was introduced into the soil in amounts which correspond to concentrations of fluoride: 0, 1, 10, 100 and 1000 mg F^-·kg^-1 d.m. soil. The second studies were conducted with additional fluoride concentrations: 200, 400, 600 and 800 mg F^-·kg^-1 d.m. soil. In the experiment two plant species were used: monocotyledonous plant – spring wheat (Triticum aestivum L.) and dicotyledonous plant – white mustard (Sinapis alba L.).
Based on the survey it was found that fluoride contamination in soil caused root growth inhibition of white mustard (Sinapis alba L.) and spring wheat (Triticum aestivum L.). Only 1 mg F^-·kg^-1 added to soil stimulated the roots length of spring wheat. The concentration of fluoride which caused 50% inhibition of root growth was 441 mg F^-·kg^-1 (in case of white mustard) and 503 mg F^-·kg^-1 (in case of spring wheat).

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