Nanocapsules are colloidal systems (r < 100nm), composed of either solid or liquid core surrounded by a polymer membrane (shell). Emulsion-core nanocapsules provide good environment for encapsulation, protection and delivery of poorly water soluble drugs improving their in vivo bioavailability and efficiency while reducing undesirable side effects. Therefore, encapsulation of therapeutic agents inside biocompatible nanocarriers is among the major research topics in nanopharmacology and nanomedicine. 

Biocompatibility of nanoproducts is the principial prerequisite for nanomedicine applications, therefore, the aim of this work was to synthesize polyelectrolyte-coated nanocapsules with emulsion cores by the layer-by-layer (LbL) technique, as described by Szczepanowicz et al [1],[2], and to examine the biocompatibility of synthesized nanocapsules with  human neuroblastoma SH-SY5Y cells. Nanoemulsions were stabilized by polyelectrolyte-surfactant complexes withsuitable surface properties. Chemicals used in nanocapsules preparation were docusate sodium salt (AOT), isopropyl myristate (IPM), chloroform (HCCl3) and various polyelectrolytes: PLL – poly(L-lysine hydrobromide), PAH – poly(allylamine hydrochloride), PDADMAC – poly(diallyl dimethyl ammonium chloride)], PGA – poly(L-glutamic acid) sodium salt, ALG – alginic acid sodium salt, CHI – chitosan, PSS – poly(sodium 4-styrenesulfonate). Prepared nanocapsules were characterized for particle size and electrical charge of successive layers adsorbed on the emulsion core by measuring their hydrodynamic diameter (DLS technique) and zeta potential (LDE technique), respectively. The biocompatibility of synthesized nanocapsules on human neuroblastoma SH-SY5Y cells using biochemical assays (MTT, LDH) was examined. Nanocapsules were tested in various concentrations and for different times of incubation.

The results obtained show that desired concentrations of several nanocapsules are non-toxic to SH-SY5Y cells and they can be used as drug-loaded nanocarriers. As the next step, the neuroprotective potential of nanoencapsulated model drugs will be evaluated against oxidative stress cytotoxity (e.g. H₂O₂ induced) in the same cell culture.

[1] Szczepanowicz, K. et al. Encapsulation of liquid cores by layer-by-layer adsorption of polyelectrolytes. Journal of Microencapsulation 27, 198-204 (2010).

[2] Szczepanowicz, K. et al. Formation of biocompatible nanocapsules with emulsion core and pegylated shell by polyelectrolyte multilayer adsorption. Langmuir 26, 12592-12597 (2010).

The work was financed by the Interdisciplinary PhD Studies "Molecular sciences for medicine" (co-financed by the European Social Fund within the Human Capital Operational Programme).

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/28291 must be provided.

1. Microelectrode surface modification with carbonaceous nanostructured hybrid composite sensing layers
2. NEW PERSPECTIVES ON THE TREATMENT OF NEURODEGENERATIVE DISEASES
3. Effect of Support Material and Conditioning on Wettability of PAH/PSS Multilayer Films