The aim of this project was to study the prevalence and severity of dental fluorosis , bone fluorosis and skelton fluorosis among persons exposed high fluoride drinking water in Assam, India  to assess the risk factors involved. Subjects aged 5 to 50 years who had been lifelong consumers of high-fluoride groundwater (> or = 10-22 mg F/L) were selected for the study. The ground water samples were collected in three seasons over one year in 2010- 2011 from 50 different sources in 30 locations on the predominantly rural and tribal area Hojai Sub-division of Nagaon district, Assam, India. The nature of sources  with their approximate depth were tube well, ring well, pond and P.H.E.water Fluoride concentrations in the water samples were determined using SPADNS method. The absorbance measurements were done at 570 nm with a cell of width 1cm using a UV spectrophotometer (Hitachi 3210). Fluoride concentration was read directly by operating the instrument in photometry mode calibrating against a standard and a blank. Among the consumers of high-fluoride water nearly100% showed dental fluorosis and 80% in the group consuming   high-fluoride water were suffering from bone fluorosis and Skelton fluorosis. Sources, distribution and geochemistry of fluoride is also studied.   In the study area, which was characterised by multiple fluoride sources uncontrolled groundwater  high fluoride content was the most important factor in the development of  dental fluorosis , bone fluorosis and skelton fluorosis.  In order to prevent  dental fluorosis , bone fluorosis and skelton fluorosis groundwater wells should routinely be analysed for fluoride.The fluoride concentration in the ground were also studied with the help of  correlation between fluoride ion and other ions. The ground water in various part of Hojai Sub-Division is highly contaminated with fluoride.Fluorosis diseases among  the children and the adults were shown through photographs.  The only way to prevent this disease is to stop the consumption of fluoride contaminated Children examined in 2010-2011were at higher risk for both dental fluorosis and bonefluorosis . This analysis showed that the risk of developing dental fluorosis, bone fluorosis and skeleton fluorosis among the children and the adults of  tribal  communities. Continuous exposure to water fluoridation had an observable effect on dental fluorosis, bone fluorosis and skeleton fluorosis . .

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