Electrochemical sensors found many applications as simple and selective analytical devices allowing fast and low-cost analysis in clinical diagnostics, environmental and process monitoring without the necessity of sample preparation. Their selectivity is mainly governed by the analyte recognition process – selective complexation of the analyte by the receptor molecules – occurring in the receptor layer. Therefore, the development of novel sensors requires the synthesis of selective receptors which could be introduced into polymeric films or immobilized on the surface of the transducers to form selective receptor layers.

Phenylboronic acid derivatives are widely used as selective receptors for developing new analytical methods for the determination of bioanalytes such as: sugars, neurotransmitters and some anions. Their high stability and especially their ability of fast and reversible ester formation with 1,2- and 1,3-diols have been applied to detect saccharides in solution (homogenous methods) as well as to design new optical saccharide sensors. In such approaches, fluorescent phenylboronic acids are usually applied to signal the binding process. Moreover, thiol-modified phenylboronic acids can be immobilized directly on the gold surface and form a self-assembled monolayer, acting as receptor layer of electrochemical sensors sensitive to sugars [1].

In this report, preliminary studies on the fabrication of electrochemical microsensors based on phenylboronic acid self-assembled monolayers of 4-mercaptophenyloboronic acid (MPBA) are presented. The efficiency of the formation of MPBA monolayers on the surface of back-side contact gold microelectrodes developed in silicon technology were studied using quartz crystal microbalance and chosen electrochemical techniques (cyclic voltammetry, impedance spectroscopy). Finally, the responses of the phenylboronic acid monolayer-modified microsensors towards selected bioanalytes (fluoride anion) have been reported.

[1] Takahashi S., Anzai J., Langmuir 21 (2005) 5102-5107

Acknowledgment

This work has been supported by the European Union in the framework of European Social Fund through the Warsaw University of Technology Development Programme, realized by Center for Advanced Studies and  by National Center for Research and Development within a framework of LIDER programme (Nr LIDER/17/202/L-1/09/NCBiR/2010) and by Project MNS-DIAG Nr POIG.01.03.01-00-014/08-00.

• Legal notice:
  

  Copyright (c) Pielaszek Research, all rights reserved.
  The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
  In every case, proper references including Author(s) name(s) and URL of this webpage: https://science24.com/paper/25043 must be provided.

1. Identification of pure pharmaceutical substance (ibuprofen) and its modification masking substances with the use of electronic tongue
2. The investigation of masking effect of Eudragite with ISE array
3. Anaerobic digestion monitoring  - application of flow-through sensor array