Comparison of techniques for determination of nanoparticle diameter

Peter Lamberg 1Marius Dagys 2Javier Sotres 1Thomas Arnebrant 1Tautgirdas Ruzgas 1

1. Malmö Univerity Health and Society, None, Malmö 20506, Sweden
2. Vilneus University, Institute of Biochemistry, Mokslininkų str. 12, Vilnius 08662, Lithuania

Abstract
Nanoparticles are studied in different fields of sciences [1]. In our laboratory we exploit gold nanoparticles (AuNPs) to promote direct electron transfer between planar electrodes and enzymes [2]. In all studies it is important to know the average size of nanoparticles (NPs). In this study we compared several techniques for NP size determination, specifically, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM) and Surface Plasmon Absorbance (SPA). Nine batches of AuNPs were prepared by well known citrate reduction method with average NP diameter ranging between 15 and 100 nm [3]. The results are summarized in the figure below.

Fig. Average diameters of nine AuNP batches measured with DLS, SPA, SEM and AFM.

The four different techniques yielded AuNP diameters with systematic differences. SEM showed the largest and SPA the smallest diameter for the same batch of AuNPs. The discrepancy in NP diameter could be because the mentioned techniques measure different aspects of the NPs. The main conclusion was that using several techniques to estimate NP size might provide a better understanding about their size and shape.

REFERENCES

  1. Whyman, R., Gold nanoparticles a renaissance in gold chemistry, 1996. Gold Bulletin 29(1): p. 11-15.
  2. Dagys, M., et al., Laccase-gold nanoparticle assisted bioelectrocatalytic reduction of oxygen, 2010. Electrochemistry Communications 12(7): p. 933-935.
  3. Turkevich, J., P.C. Stevenson, and J. Hillier, A study of the nucleation and growth processes in the synthesis of colloidal gold, 1951. Discussions of the Faraday Society 11: p. 55-75.

Legal notice
  • Legal notice:

    Copyright (c) Pielaszek Research, all rights reserved.
    The above materials, including auxiliary resources, are subject to Publisher's copyright and the Author(s) intellectual rights. Without limiting Author(s) rights under respective Copyright Transfer Agreement, no part of the above documents may be reproduced without the express written permission of Pielaszek Research, the Publisher. Express permission from the Author(s) is required to use the above materials for academic purposes, such as lectures or scientific presentations.
    In every case, proper references including Author(s) name(s) and URL of this webpage: http://science24.com/paper/24876 must be provided.

 

Related papers
  1. Electron Transfer in Complex Two-cofactor-containing Enzymes at Alkanethiol-modified Gold Electrodes

Presentation: Short communication at SMCBS'2011 International Workshop, by Peter Lamberg
See On-line Journal of SMCBS'2011 International Workshop

Submitted: 2011-08-24 11:01
Revised:   2011-08-24 11:04
Google
 
Web science24.com
© 1998-2021 pielaszek research, all rights reserved Powered by the Conference Engine